UV-1200 UV-visible spectrophotometer hydrogen sulfide - methylene blue spectrophotometry

Hydrogen sulfide-methylene blue spectrophotometry

Key words: methylene blue spectrophotometry; hydrogen sulfide; mass spectrometer ; UV-1100; UV-1200 $Src="http://i.bosscdn.com/blog/nophoto.gif" />1. Pipette 5mL of acidic water sample into a 1000mL volumetric flask, dilute to the mark, shake to be tested (purified water does not need to be diluted) . 2. Pipette 2 mL of the sample from the dilution solution into a centrifuge tube containing 10 mL of borax buffer solution in advance, and add 20 mL of zinc acetate-acetic acid. Sodium, mix and place in a centrifuge (remember the number). (Purified water is taken from the original water sample 10mL in...) 3. Start the centrifuge and centrifuge for 5 minutes. 4. Remove the centrifuge tube, discard the supernatant, retain the pellet, transfer all the pellet to a 100 mL colorimetric tube, and add water to 80 mL. 5. Add 10mL 2g/L of N-N-dimethyl-p-phenylenediamine solution along the wall of the tube and immediately shake it to avoid the loss of hydrogen sulfide and make the precipitate completely dissolved. 6. Add 1 mL of ammonium ferric sulfate solution along the tube wall, and dilute to 100 mL. Immediately shake and shake. After standing for 10 min, use a 1 cm cuvette at 665 nm of the spectrophotometer. . 7. At the same time, replace the blank test with distilled water. 8. Calculate the concentration of hydrogen sulfide in acidic water (mg/L): the concentration of hydrogen sulfide in purified water (mg/L): C - direct reading result of spectrophotometer Keywords: methylene blue spectrophotometry; hydrogen sulfide; Macylab.com;UV-1100;UV-1200</p> </div> <div class="tech-detail-share">  <div class="bdsharebuttonbox"> a href="#" class="bds_qzone" data-cmd="qzone" title="Share to QQ space"></a> <a href="#" class="bds_tsina" data-cmd="tsina" Title="Share to Sina Weibo"></a> <a href="#" class="bds_weixin" data-cmd="weixin" title="Share to WeChat"></a> <span>Share to :</span> </div> <script>window._bd_share_config = { "common": { "bdSnsKey": {}, "bdText": "", "bdMini": "1", "bdMiniList": false, "bdPic": "", "bdStyle": "2", "bdSize": "16" }, "share": {} }; with (document) 0[ (getElementsByTagName($

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