Analysis of different dilutions of samples in Elisa assay - Database & Sql Blog Articles

EL-C1600N100013-B

Test - lowercase jpg

Kaixin micro test

Test probe P100-M3

According to our experience, the commonly used modes of ELISA assay: indirect method test antibody, competition method test antibody, competition method test antigen, double antibody sandwich method test antigen, double antigen sandwich assay antibody. The dilution of the sample in the experiment is the most important. A little carelessness leads to a loss of all. Today, we will list the specific sample dilution principles for you:
General Principles of Sample Dilution The user must estimate the amount of the test factor to be determined and determine the appropriate dilution factor so that the concentration of the test factor in the diluted sample is within the optimal detection range of the elisa kit. According to the high, medium and low levels of the factors to be tested, different dilution schemes are adopted:
High - means the factor to be measured is between 20 and 200 ng/ml. Usually diluted 1:100. 297 ul of sample dilution plus 3 ul of sample.
Medium - means the factor to be measured is 2-20 ng/ml. Generally diluted 1:10. 225 ul of sample dilution plus 25 ul of sample.
Low - means that the factor to be tested is between 31.2 and 2000 pg/ml. Dilute 1:2. 100 ul of sample dilution plus 100 ul of sample.
Extra low - refers to the factor to be measured ≤ 31.2pg / ml. Samples are generally not diluted and added directly to 100 ul of sample per well. The above scheme is for reference only and the dilution of the sample should be recorded in detail.
The above is the full content of the sample dilution in this chapter. If you have any questions that you don't understand, you can call us. Shanghai Jinma Bio welcomes you.